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Below is a cache of http://www.southernbiotech.com/techbul/10040.pdf. It's a snapshot of the page taken as our search engine crawled the Web.
The web site itself may have changed. You can check the current page or check for previous versions at the Internet Archive. Yahoo! is not affiliated with the authors of this page or responsible for its content. ApoScreen Annexin V Corporate Offices: 160A Oxmoor Blvd. Birmingham, AL 35209 USA Mailing Address: P.O. Box 26221 Birmingham, AL 35260 USA Tel: 205.945.1774 U.S. and Canada: 800.722.2255 Fax: 205.945.8768 Email: info@southernbiotech.com Website: www.southernbiotech.com ApoScreen Annexin V Cat. No. Form Quantity
10040-02 Annexin V Fluorescein (FITC) Conjugate 100 tests 10040-08 Annexin V Biotin (BIOT) Conjugate 100 tests 10040-09 Annexin V R-phycoerythrin (R-PE) Conjugate 100 tests 10040-11 Annexin V Allophycocyanin (APC) Conjugate 100 tests 10040-15 Annexin V *Cyanine 5 (CY 5) Conjugate 100 tests 10040-22 Annexin V Fluorescein-5-Maleimide (FLMA) Conjugate 100 tests DESCRIPTION
A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. 1 Annexin V (Mr 36-kDa), a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for PS-containing phospholipid bilayers. 2,3 When PS is exposed on the extracellular face of a cell membrane, annexin V binds with an affinity of approximately 7 nM. 4 Fluorochrome conjugates of annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, 5-8 thereby providing a convenient tool for detection of apoptotic cells. ApoScreen Annexin V is a labeled annexin V that can be used in conjunction with propidium iodide to evaluate subpopulations of cells along the apoptotic pathway. ApoScreen detection reagents can also be used to analyze changes in membrane asymmetry in platelets 5 and erythrocytes. 8 Analysis can be performed on a flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells can be examined with a fluorescence microscope equipped with appropriate filters.
RESEARCH APPLICATIONS
Blocking Annexin V-conjugates
Flow cytometry Fluorescence microscopy
CHARACTERIZATION To insure acceptable performance, each batch of product is tested by flow cytometry to conform to the
characteristics of a standard reference reagent.
WORKING DILUTIONS Flow Cytometry: Fluorescein conjugates
Biotin conjugate
R-phycoerythrin conjugate
Allophycocyanin conjugate
Cyanine 5 conjugate 10
The web site itself may have changed. You can check the current page or check for previous versions at the Internet Archive. Yahoo! is not affiliated with the authors of this page or responsible for its content. ApoScreen Annexin V Corporate Offices: 160A Oxmoor Blvd. Birmingham, AL 35209 USA Mailing Address: P.O. Box 26221 Birmingham, AL 35260 USA Tel: 205.945.1774 U.S. and Canada: 800.722.2255 Fax: 205.945.8768 Email: info@southernbiotech.com Website: www.southernbiotech.com ApoScreen Annexin V Cat. No. Form Quantity
10040-02 Annexin V Fluorescein (FITC) Conjugate 100 tests 10040-08 Annexin V Biotin (BIOT) Conjugate 100 tests 10040-09 Annexin V R-phycoerythrin (R-PE) Conjugate 100 tests 10040-11 Annexin V Allophycocyanin (APC) Conjugate 100 tests 10040-15 Annexin V *Cyanine 5 (CY 5) Conjugate 100 tests 10040-22 Annexin V Fluorescein-5-Maleimide (FLMA) Conjugate 100 tests DESCRIPTION
A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. 1 Annexin V (Mr 36-kDa), a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for PS-containing phospholipid bilayers. 2,3 When PS is exposed on the extracellular face of a cell membrane, annexin V binds with an affinity of approximately 7 nM. 4 Fluorochrome conjugates of annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, 5-8 thereby providing a convenient tool for detection of apoptotic cells. ApoScreen Annexin V is a labeled annexin V that can be used in conjunction with propidium iodide to evaluate subpopulations of cells along the apoptotic pathway. ApoScreen detection reagents can also be used to analyze changes in membrane asymmetry in platelets 5 and erythrocytes. 8 Analysis can be performed on a flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells can be examined with a fluorescence microscope equipped with appropriate filters.
RESEARCH APPLICATIONS
Blocking Annexin V-conjugates
Flow cytometry Fluorescence microscopy
CHARACTERIZATION To insure acceptable performance, each batch of product is tested by flow cytometry to conform to the
characteristics of a standard reference reagent.
WORKING DILUTIONS Flow Cytometry: Fluorescein conjugates
Biotin conjugate
R-phycoerythrin conjugate
Allophycocyanin conjugate
Cyanine 5 conjugate 10
